Bacteria Lysing Bead Mill Tubes

Product Number: 30391402 (100 each)

Contains: 100 μm Zirconium Beads

Button Setting #1: 5 min. @ 1500 rpm

Protocol:

• Spin down 2 ml of sample or cultured bacteria.
• Decant the supernatant and resuspend the pellet in 500 μl of a suitable buffer.
• Transfer the solution to a Bacteria Lysis Bead Tube.
• Homogenize for 5 minutes at 1500 rpm (press preset button).
• Centrifuge for 5 minutes at 12,000 × g.
• Transfer the supernatant to a clean tube and store/process.

May be used for: bacteria/spores

Helpful Hints:

• Increase the homogenization time if obtaining low DNA concentrations from gram positive bacteria.
• Assays can be performed to assess optimal homogenization conditions.