- GST No. 27AAACO8629G1Z9
Bead Mill Tubes
Leading Manufacturers, Exporters, Wholesaler, Retailer and Trader of Bacteria Lysing Bead Mill Tubes, Soft Sample Lysing Bead Mill Tubes and Yeast Lysing Bead Mill Tubes from Mumbai.
| Business Type | Manufacturer, Exporter, Supplier |
| Brand Name | Ohaus |
| Usage | Lab |
Preferred Buyer From
| Location | Worldwide |
Product Number: 30391402 (100 each)
Contains: 100 μm Zirconium Beads
Button Setting #1: 5 min. @ 1500 rpm
Protocol:
- Spin down 2 ml of sample or cultured bacteria.
- Decant the supernatant and resuspend the pellet in 500 μl of a suitable buffer.
- Transfer the solution to a Bacteria Lysis Bead Tube.
- Homogenize for 5 minutes at 1500 rpm (press preset button).
- Centrifuge for 5 minutes at 12,000 × g.
- Transfer the supernatant to a clean tube and store/process.
May be used for: bacteria/spores
Helpful Hints:
- Increase the homogenization time if obtaining low DNA concentrations from gram positive bacteria.
- Assays can be performed to assess optimal homogenization conditions.
| Business Type | Manufacturer, Exporter, Supplier |
| Brand Name | Ohaus |
| Usage | Lab |
Preferred Buyer From
| Location | Worldwide |
Product Number: 30391406 (100 each)
Contains: 1.4 mm Zirconium Beads
Button Setting#2: 2 min. @ 1500 rpm
Protocol:
- For cultured cells or blood, centrifuge sample and decant supernatant. Resuspend the pellet in 500 μl of a suitable buffer and transfer to a Lysing Bead Tube. For solid samples, place approximately 50 mg in a Lysing Bead Tube and 500 μl of a suitable buffer. Homogenize for 2 minutes at 1500 rpm (press present button).
- Remove debris by centrifuging for 5 minutes at 12,000 × g.
- Transfer the supernatant to a clean tube for processing or storage.
May be used for: soft tissues (e.g., brain, adipose, liver, spleen); cultured cells and blood, as well as thin sections of fungal thallus and soft plant material
| Business Type | Manufacturer, Exporter, Supplier |
Preferred Buyer From
| Location | Worldwide |
Product Number: 30391404 (100 each)
Contains: 400 μm Zirconium Beads
Button Setting #1: 5 min. @ 1500 rpm
Protocol:
- Spin down 1 ml of sample or cultured yeast.
- Decant supernatant and resuspend the pellet in 500 μl of a suitable buffer.
- Transfer the yeast solution to a Yeast Lysing Bead Tube.
- Homogenize for 5 minutes at 1500 rpm (press preset button).
- To remove debris, centrifuge for 5 minutes at 12,000 × g.
- Transfer the supernatant to a clean tube for processing or storage.
May be used for: yeast, algae, spores
Helpful Hints:
- Longer processing times may be required to homogenize smaller yeasts (i.e. Pichia).
- Homogenization can be evaluated by comparing homogenized yeast to a non-homogenized sample under a microscope. Yeast cells that are cracked open will appear dark gray.
