Yeast Lysing Bead Mill Tubes

Product Number: 30391404 (100 each)

Contains: 400 μm Zirconium Beads

Button Setting #1: 5 min. @ 1500 rpm

Protocol:

• Spin down 1 ml of sample or cultured yeast.
• Decant supernatant and resuspend the pellet in 500 μl of a suitable buffer.
• Transfer the yeast solution to a Yeast Lysing Bead Tube.
• Homogenize for 5 minutes at 1500 rpm (press preset button).
• To remove debris, centrifuge for 5 minutes at 12,000 × g.
• Transfer the supernatant to a clean tube for processing or storage.

May be used for: yeast, algae, spores

Helpful Hints: 

• Longer processing times may be required to homogenize smaller yeasts (i.e. Pichia).
• Homogenization can be evaluated by comparing homogenized yeast to a non-homogenized sample under a microscope. Yeast cells that are cracked open will appear dark gray.